Real-Time PCR Detection System

Scope of Work: The UDSA-ARS Nation Center for Cool and Cold-Water Aquaculture Research Unit out of the Great Lakes Water Institute (University of Wisconsin Milwaukee’s School of Freshwater Sciences) in Milwaukee, WI is seeking sources for a small footprint real-time PCR detection system or the equivalent. Specifications for the purchase are itemized below. Background: The USDA-ARS Milwaukee Aquaculture Program uses the real-time detection system to measure quantitative levels of gene abundances (messenger RNA) in tissues of rainbow trout to characterize how genes involved with immune function are regulated. Technical Requirements: The Real-time PCR detection system will be used routinely to add analytical capacity for measurement of immune gene expression in rainbow trout. Small footprint detection system, with integrated touch screen capable of running instrument. Software is intuitive, convenient & flexible with unlimited number of licenses, enabling: Comparative Ct, Standard Curve, Relative Standard Curve, Genotyping, Presence/Absence, Melt Curve Analyses. Multicomponenting algorithm for optimal dye resolution. 96-well block, compatible with fast cycling or standard conditions/chemistries. No loss of multiplex-capabilities when running fast chemistries and cycling parameters. High-performance block cycling system with a wide programmable temperature range from 4°C to 100°C with flexible block technology to run independent Peltier blocks for 3 independent zones for better assay optimization and temperature zone precision & better than gradient reaction optimization. LED-based detection system, capable of simultaneous detection of 4 different dyes. LED system is pre-calibrated 4-color white LED optical system. For on-site set up a calibration, system comes with a free RNaseP Verification Plate. Real-time integration & normalization of assays using chemistries with ROX as a passive reference dye. USB ports for convenient data download/upload on the instrument Simplified recalibration-option to guarantee optimal performance even PC/MAC computer compatible (using web browser-based software) Option to collect all filters for all wells regardless of plate setup enabling post-run setup. Multiplate Data Comparison—Compare an unlimited number of plates of gene expression assays. RDML export for compatibility with MIQE guidelines. Works with a wide variety of co-optimized reagents, such as TaqMan® assay portfolio and SYBR chemistries (all with ROX passive reference dye) for high performance of both, routine and challenging applications. Free optional Smart Monitor service to maximize system uptime and improve productivity. cUL (tested to CAN/CSA standards), UL, CE, C-TICK, RoHS/WEEE compliant. Optional: access to knowledgeable team of technical application scientists is available to assist with real-time PCR questions by phone or email with flexible service and training plans to fit specific laboratory requirements. Half day training – SmartStart Mfr. Design and Analysis Web Browser-based Software Mfr. Design and Analysis Desktop Software LIMITED WARRANTY: Two-year parts and labor Shipping must be included. Laptop Computer Specifications: Laptop computer with control & analytical software. Control system should be compatible with a computer or tablet of the following specifications. OS: Microsoft® Windows® 10 (64-bit) CPU: Intel® Core TM i3 (2.1 GHz) or more RAM: 4 GB or more HDD: Free space: 2 GB or more Screen resolution: 1366 × 768 or more Web Browser: Google Chrome TM Vendor will work closely with ARS-USDA-IT to ensure computer/tablet complies with Government security requirements. Warranty: Two (2) years parts & labor on system components Brand Name or Equal: ABI QuantStudio 3 Real-Time PCR Detection System Customer will provide access to movers to complete installation, planned maintenance, Installation Performance Verification, and other service calls within the warranty plan period. Customer will provide no formal direction to vendor but will provide access to stored tools and other laboratory sites needed to perform service. Vendor determines which service engineer to send for site visits or repair. Key Deliverables: Real-Time PCR Detection System Laptop Software Delivery Instillation Training

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